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2013). These multilamellar bodies presented an internal compartment with fibrillar material, equivalent

(D) Disc-shaped plastid using a slight central depression (arrow). (E) Plastid beginning to engulf cytoplasm. The arrow points to a deep depression thatcreates a cytoplasmic pocket inside the plastid. (F,F') Cytoplasm-containing plastid where the internal cytoplasm is connected using the outer cytoplasm just by a narrow channel that ends within a compact pore at the plastid surface. Altogether, these plastid profiles suggested the occurrence of plastid degradation and excretion out of the cell.3-D RECONSTRUCTION OF SUBCELLULAR VOLUMES OF EMBRYOGENIC MICROSPORESTable 1 | Quantitative evaluation of plastids of embryogenic microspores. Quantity Percentage Percentage (from total) (from atypical) Standard Atypical Engulfing (open profiles) Engulfed (closed profiles) Concentric membranes/disorganized contents/multilamellar Total 142 92 14 63 15 60.7 39.three six.0 26.9 6.415.two 68.5 16.3100100Theoretically, it could be possible that the atypical plastid profiles observed in TEM micrographs of embryogenic microspores correspond to polar sections of cup-shaped plastids. Alternatively, these plastid profiles may well correspond to equatorial sections of ring-shaped plastids. In other words, the atypical plastid profiles we observed might be artifactual, and <a href='https://dx.doi.org/10.1073/pnas.1015994108 title='View abstract' target='resource_window'>pnas.1015994108 might not engulf cytoplasm basically. To be able to rule out this possibility, and to figure out the actual 3-D structure of those plastids, we performed FESEM-FIB-based 3-D reconstructions and models of huge cytoplasmic places of embryogenic microspores (Figure 4A; Supplementary Film S1). These models confirmed the presence of 3 morphologically distinctive plastid typesFIGURE 4 | 3-D model of a subcellular volume of a B. napus embryogenic microspore. (A) Modeled subcellular volume. (B) Model excluding all the cell structures however the plastids (pl). The diverse plastid forms are modeled in various colors: traditional (light green), open profiles engulfing cytoplasm (dark green), and closed profiles (yellow) using the engulfed cytoplasm (white). (C) Traditional, round-shaped plastid. (D) Disc-shaped plastid having a slight central depression (arrow). (E) Plastid beginning to engulf cytoplasm. The arrow points to a deep depression thatcreates a cytoplasmic pocket inside the plastid. (F,F') Cytoplasm-containing plastid where the internal cytoplasm is connected together with the outer cytoplasm just by a narrow channel that ends inside a tiny pore at the plastid surface. (F') is actually a 90 turn of this plastid, to get a clear visualization of the narrow channel. PF-04418948 web Arrows point to <a href='https://dx.doi.org/10.1073/pnas.1107775108 title='View abstract' target='resource_window'>pnas.1107775108 the pore in (F) and to the narrow channel in (F'). (G) Round plastid (yellow) with the cytoplasmic contents (white) totally isolated from the outer cytoplasm. cw, cell wall; m, mitochondrion; n, nucleus.2013). These multilamellar bodies presented an internal compartment with fibrillar material, equivalent to that present in lytic compartments. Closed plastid profiles with concentric membranes, dark, fibrillar, and disorganized contents, together with cytoplasmic and apoplastic multilamellar bodies, accounted for 16.3 with the atypical profiles observed. Altogether, these plastid profiles suggested the occurrence of plastid degradation and excretion out on the cell.3-D RECONSTRUCTION OF SUBCELLULAR VOLUMES OF EMBRYOGENIC MICROSPORESTable 1 | Quantitative analysis of plastids of embryogenic microspores. Number Percentage Percentage (from total) (from atypical) Traditional Atypical Engulfing (open profiles) Engulfed (closed profiles) Concentric membranes/disorganized contents/multilamellar Total 142 92 14 63 15 60.7 39.3 6.0 26.9 6.415.2 68.five 16.3100100Theoretically, it could be probable that the atypical plastid profiles observed in TEM micrographs of embryogenic microspores correspond to polar sections of cup-shaped plastids.
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